S Tier
Snake

A Tier
Falco
King Dedede
Mr. Game & Watch
Marth
Diddy Kong

B Tier
Wario
R.O.B.
Lucario
Olimar

C Tier
Pikachu
Kirby
Donkey Kong
Ice Climbers

D Tier
Zero Suit Samus
Toon Link
Pit
Peach
Wolf

E Tier
Luigi
Zelda
Bowser
Fox
Sheik
Ike

F Tier
Mario
Lucas
Ness
Samus
Sonic
Pokémon Trainer
Yoshi

G Tier
Link
Jigglypuff
Ganondorf
Captain Falcon

Added (06.17.2009, 7:19 Am)
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well this forum was pretty much a bust but i'm bored so i'm gonna update the list XD

S Tier:
Meta Knight
Snake
Wario
Falco
Diddy Kong
King Dedede

A Tier:
Marth
Mr. Game & Watch
Pikachu
Olimar
Ice Climbers
R.O.B.
Kirby

B Tier:
Lucario
Zero Suit Samus
Toon Link
Pit
Donkey Kong

C Tier:
Peach
Luigi
Fox
Wolf
Sonic
Sheik

D Tier:
Bowser
Zelda
Pokémon Trainer
Ike

E Tier:
Lucas
Mario
Ness
Yoshi
Samus

F Tier:
Jigglypuff
Captain Falcon
Link
Ganondorf